Biochemistry Question
Learning Goal: I’m working on a biochemistry question and need support to help me learn.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine.
| Enzyme | Target sequence | Cleavage |
|---|---|---|
| EcoRI | 5′ GAATTC 3′ 3′ CTTAAG 5′ | 5′ G AATTC 3′ 3′ CTTAA G 5′ |
| EcoRV | 5′ GATATC 3′ 3′ CTATAG 5′ | 5′ GAT ATC 3′ 3′ CTA TAG 5′ |
| HaeIII | 5′ GGCC 3′ 3′ CCGG 5′ | 5′ GG CC 3′ 3′ CC GG 5′ |
| HindIII | 5′ AAGCTT 3′ 3′ TTCGAA 5′ | 5′ A AGCTT 3′ 3′ TTCGA A 5′ |
| PpuMI | 5′ RGGWCCY 3′ 3′ YCCWGGR 5′ | 5′ RG GWCCY 3′ 3′ YCCWG GR 5′ |
Which restriction endonuclease(s) would cleave a DNA molecule at the given sequences?a) 5′ TCAGAATTCGGTGA 3’ECORV
ECORI
HINDIII
HAEIIIb) 5′ AGGCCGAATTCGC 3’ECORI
ECORV
HAEIII
HINDIII
c) Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for the sticky end of the gene in the 5′ to 3′ direction.
possible sequence of bases:
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